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Home / Cell /Experiment / 培养腹膜微血管内皮细胞大概步骤

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培养腹膜微血管内皮细胞大致步骤:
剪取富含血管的大网膜组织,去除大部分脂肪,剪碎,1型胶原酶消化30分钟,200目筛网过滤,

离心滤过液,弃去上清液,悬浮沉积物,500目筛网再滤过,

微血管段遗留于筛网上,冲洗筛网,收集冲洗液离心,沉积物为微血管段,

培养液悬浮放入培养瓶培养,内皮细胞24-36小时后从微血管段爬出,

换液弃去微血管段开始培养内皮细胞。

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